biostudies-literatureUnknown13Ding Z<h4>Background</h4>Affinity precipitation has been reported as a potential technology for the purification of proteins at the early stage of downstream processing. The technology could be achieved using reversible soluble-insoluble polymers coupled with an affinity ligand to purify proteins from large volumes of dilute solution material such as fermentation broths or plasma. In this study, a thermo-response polymer was synthesized using N-methylol acrylamide, N-isopropyl acrylamide and butyl acrylate as monomers. The molecular weight of the polymer measured by the viscosity method was 3.06 × 104 Da and the lower critical solution temperature (LCST) was 28.0°C.The recovery of the polymer above the LCST was over 95.0%. Human serum albumin (HSA) is the most abundant protein in the human serumBMC biotechnology109https://www.ebi.ac.uk/biostudies/studies/S-EPMC3903448biostudies-literatureAffinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand.PMC3903448Cao XDing ZfalseAffinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand.<h4>Background</h4>Affinity precipitation has been reported as a potential technology for the purification of proteins at the early stage of downstream processing. The technology could be achieved using reversible soluble-insoluble polymers coupled with an affinity ligand to purify proteins from large volumes of dilute solution material such as fermentation broths or plasma. In this study, a thermo-response polymer was synthesized using N-methylol acrylamide, N-isopropyl acrylamide and butyl acrylate as monomers. The molecular weight of the polymer measured by the viscosity method was 3.06 × 104 Da and the lower critical solution temperature (LCST) was 28.0°C.The recovery of the polymer above the LCST was over 95.0%. Human serum albumin (HSA) is the most abundant protein in the human serum2013-01-01T00:00:00Z2013 Dec2025-04-21T20:00:31.424Z2019-03-27T01:20:42ZS-EPMC39034482434131510.1186/1472-6750-13-109