{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lormand JD"],"funding":["NIEHS NIH HHS","NIGMS NIH HHS"],"pagination":["10323-33"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3905856"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(22)"],"pubmed_abstract":["Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and progression by the replicative DNA polymerase δ/proliferating cell nuclear antigen/replication factor C complex on telomeric templates that mimic the leading C-rich and lagging G-rich strands. Increased polymerase stalling occurred on the G-rich template, compared with the C-rich and nontelomeric templates. Suppression of G4 formation by substituting Li(+) for K(+) as the cation, or by using templates with 7-deaza-G residues, did not alleviate Pol δ pause sites within the G r"],"journal":["Nucleic acids research"],"pubmed_title":["DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation."],"pmcid":["PMC3905856"],"funding_grant_id":["R01GM031973","R01ES0515052","R00ES016758","R01 GM065484","R00 ES016758","R01 ES015052","R01GM032431","Z01 ES065070"],"pubmed_authors":["Opresko PL","Burgers P","Kunkel TA","Lormand JD","Kaur P","Wang H","Lee MY","Buncher N","Murphy CT"],"additional_accession":[]},"is_claimable":false,"name":"DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation.","description":"Previous evidence indicates that telomeres resemble common fragile sites and present a challenge for DNA replication. The precise impediments to replication fork progression at telomeric TTAGGG repeats are unknown, but are proposed to include G-quadruplexes (G4) on the G-rich strand. Here we examined DNA synthesis and progression by the replicative DNA polymerase δ/proliferating cell nuclear antigen/replication factor C complex on telomeric templates that mimic the leading C-rich and lagging G-rich strands. Increased polymerase stalling occurred on the G-rich template, compared with the C-rich and nontelomeric templates. Suppression of G4 formation by substituting Li(+) for K(+) as the cation, or by using templates with 7-deaza-G residues, did not alleviate Pol δ pause sites within the G r","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Dec","modification":"2025-04-22T19:27:09.299Z","creation":"2019-03-27T01:20:50Z"},"accession":"S-EPMC3905856","cross_references":{"pubmed":["24038470"],"doi":["10.1093/nar/gkt813"]}}