{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Jun EK"],"pubmed_abstract":["In a previous study, we isolated human amniotic fluid (AF)-derived mesenchymal stem cells (AF-MSCs) and utilized normoxic conditioned medium (AF-MSC-norCM) which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM), it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials). Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM) compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the pro"],"journal":["International journal of molecular sciences"],"pagination":["605-28"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3907828"],"repository":["biostudies-literature"],"pubmed_title":["Hypoxic conditioned medium from human amniotic fluid-derived mesenchymal stem cells accelerates skin wound healing through TGF-β/SMAD2 and PI3K/Akt pathways."],"pmcid":["PMC3907828"],"pubmed_authors":["Park G","Kim A","Moon JH","You S","Jun EK","Lee G","Kang PJ","Zhang Q","Yoon BS","Lee JH"],"additional_accession":[]},"is_claimable":false,"name":"Hypoxic conditioned medium from human amniotic fluid-derived mesenchymal stem cells accelerates skin wound healing through TGF-β/SMAD2 and PI3K/Akt pathways.","description":"In a previous study, we isolated human amniotic fluid (AF)-derived mesenchymal stem cells (AF-MSCs) and utilized normoxic conditioned medium (AF-MSC-norCM) which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM), it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials). Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM) compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the pro","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jan","modification":"2026-04-29T15:32:21.017Z","creation":"2019-03-27T01:20:59Z"},"accession":"S-EPMC3907828","cross_references":{"pubmed":["24398984"],"doi":["10.3390/ijms15010605"]}}