{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(5)"],"submitter":["Tripathi P"],"pubmed_abstract":["Drought is the major cause of crop losses worldwide. Water stress-inducible promoters are important for understanding the mechanisms of water stress responses in crop plants. Here we utilized tobacco (Nicotiana tabacum L.) Bright Yellow 2 (BY-2) cell system in presence of polyethylene glycol, salt and phytohormones. Extension of the system to 85 mM NaCl led to inducibility of up to 10-fold with the water stress and salt responsive soybean GmWRKY53 promoter. Upon ABA and JA treatment fold inducibility was up to 5-fold and 14-fold, respectively. Thus, we hypothesize that GmWRKY53 could be used as potential model candidate for dissecting drought regulatory elements as well as understanding crosstalk utilizing a rapid heterologous system of BY-2 culture."],"journal":["Plant signaling & behavior"],"pagination":["e24097"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3909080"],"repository":["biostudies-literature"],"pubmed_title":["GmWRKY53, a water- and salt-inducible soybean gene for rapid dissection of regulatory elements in BY-2 cell culture."],"pmcid":["PMC3909080"],"pubmed_authors":["Rushton PJ","Tripathi P","Lin J","Rabara RC"],"additional_accession":[]},"is_claimable":false,"name":"GmWRKY53, a water- and salt-inducible soybean gene for rapid dissection of regulatory elements in BY-2 cell culture.","description":"Drought is the major cause of crop losses worldwide. Water stress-inducible promoters are important for understanding the mechanisms of water stress responses in crop plants. Here we utilized tobacco (Nicotiana tabacum L.) Bright Yellow 2 (BY-2) cell system in presence of polyethylene glycol, salt and phytohormones. Extension of the system to 85 mM NaCl led to inducibility of up to 10-fold with the water stress and salt responsive soybean GmWRKY53 promoter. Upon ABA and JA treatment fold inducibility was up to 5-fold and 14-fold, respectively. Thus, we hypothesize that GmWRKY53 could be used as potential model candidate for dissecting drought regulatory elements as well as understanding crosstalk utilizing a rapid heterologous system of BY-2 culture.","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 May","modification":"2025-04-18T21:59:52.506Z","creation":"2019-03-27T01:21:01Z"},"accession":"S-EPMC3909080","cross_references":{"pubmed":["23511199"],"doi":["10.4161/psb.24097"]}}