{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Iyer LK"],"funding":["NIGMS NIH HHS"],"pagination":["4629-39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3913734"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(12)"],"pubmed_abstract":["Local side-chain interactions in lyophilized protein formulations were mapped using solid-state photolytic labeling-mass spectrometry (ssPL-MS). Photoactive amino acid analogues (PAAs) were used as probes and either added to the lyophilized matrix or incorporated within the amino acid sequence of a peptide. In the first approach, apomyoglobin was lyophilized with sucrose and varying concentrations of photoleucine (L-2-amino-4,4'-azipentanoic acid; pLeu). The lyophilized solid was irradiated at 365 nm to initiate photolabeling. The rate and extent of labeling were measured using electrospray ionization/high-performance liquid chromatography/mass spectrometry (ESI-HPLC-MS), with labeling reaching a plateau at ~30 min, forming up to six labeled populations. Bottom-up MS/MS analysis was able t"],"journal":["Molecular pharmaceutics"],"pubmed_title":["Photolytic labeling to probe molecular interactions in lyophilized powders."],"pmcid":["PMC3913734"],"funding_grant_id":["NIH RO1 GM085293","R01 GM085293"],"pubmed_authors":["Iyer LK","Moorthy BS","Topp EM"],"additional_accession":[]},"is_claimable":false,"name":"Photolytic labeling to probe molecular interactions in lyophilized powders.","description":"Local side-chain interactions in lyophilized protein formulations were mapped using solid-state photolytic labeling-mass spectrometry (ssPL-MS). Photoactive amino acid analogues (PAAs) were used as probes and either added to the lyophilized matrix or incorporated within the amino acid sequence of a peptide. In the first approach, apomyoglobin was lyophilized with sucrose and varying concentrations of photoleucine (L-2-amino-4,4'-azipentanoic acid; pLeu). The lyophilized solid was irradiated at 365 nm to initiate photolabeling. The rate and extent of labeling were measured using electrospray ionization/high-performance liquid chromatography/mass spectrometry (ESI-HPLC-MS), with labeling reaching a plateau at ~30 min, forming up to six labeled populations. Bottom-up MS/MS analysis was able t","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Dec","modification":"2026-05-01T20:19:39.297Z","creation":"2019-03-27T01:21:16Z"},"accession":"S-EPMC3913734","cross_references":{"pubmed":["24125175"],"doi":["10.1021/mp4004332"]}}