biostudies-literatureUnknown22(2)Weng TYInduction of thrombospondin 1 (TSP-1) is generally assumed to suppress tumor growth through inhibiting angiogenesis; however, it is less clear how TSP-1 in dendritic cells (DCs) influences tumor progression. We investigated tumor growth and immune mechanism by downregulation of TSP-1 in dendritic cells. Administration of TSP-1 small hairpin RNA (shRNA) through the skin produced anticancer therapeutic effects. Tumor-infiltrating CD4(+) and CD8(+) T cells were increased after the administration of TSP-1 shRNA. The expression of interleukin-12 and interferon-γ in the lymph nodes was enhanced by injection of TSP-1 shRNA. Lymphocytes from the mice injected with TSP-1 shRNA selectively killed the tumor cells, and the cytotoxicity of lymphocytes was abolished by depletion of CD8(+) T cells. InjecMolecular therapy : the journal of the American Society of Gene Therapy292-302https://www.ebi.ac.uk/biostudies/studies/S-EPMC3918914biostudies-literatureA novel cancer therapeutic using thrombospondin 1 in dendritic cells.PMC3918914Chang JYHuang SSWang CYLin CMLai MDYen MCLin CCChen WCWeng TYChen YLfalseA novel cancer therapeutic using thrombospondin 1 in dendritic cells.Induction of thrombospondin 1 (TSP-1) is generally assumed to suppress tumor growth through inhibiting angiogenesis; however, it is less clear how TSP-1 in dendritic cells (DCs) influences tumor progression. We investigated tumor growth and immune mechanism by downregulation of TSP-1 in dendritic cells. Administration of TSP-1 small hairpin RNA (shRNA) through the skin produced anticancer therapeutic effects. Tumor-infiltrating CD4(+) and CD8(+) T cells were increased after the administration of TSP-1 shRNA. The expression of interleukin-12 and interferon-γ in the lymph nodes was enhanced by injection of TSP-1 shRNA. Lymphocytes from the mice injected with TSP-1 shRNA selectively killed the tumor cells, and the cytotoxicity of lymphocytes was abolished by depletion of CD8(+) T cells. Injec2014-01-01T00:00:00Z2014 Feb2026-04-29T22:36:41.379Z2019-03-27T01:21:30ZS-EPMC39189142412701010.1038/mt.2013.236