biostudies-literatureZheng TBiotechnology and Biological Sciences Research Council2182-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC3926089biostudies-literatureUnknown111(6)The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg1-1 mutants, indicating that this cdk-Proceedings of the National Academy of Sciences of the United States of AmericaCDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana.PMC3926089BBS/E/W/10962A01BBB/F018754/1BB/J007633/1BBS/E/J/0000A216BB/J004405/1Phillips DWJenkins GDoonan JHNibau CZheng TArmstrong SJfalseCDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana.The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg1-1 mutants, indicating that this cdk-2014-01-01T00:00:00Z2014 Feb2025-04-04T21:13:38.353Z2019-03-27T01:21:53ZS-EPMC39260892446982910.1073/pnas.1318460111