biostudies-literatureRosch JWNIAID NIH HHSNIDCD NIH HHSNIGMS NIH HHS141-54https://www.ebi.ac.uk/biostudies/studies/S-EPMC3936495biostudies-literatureUnknown6(1)Acute otitis media (AOM) caused by Streptococcus pneumoniae remains one of the most common infectious diseases worldwide despite widespread vaccination. A major limitation of the currently licensed pneumococcal vaccines is the lack of efficacy against mucosal disease manifestations such as AOM, acute bacterial sinusitis and pneumonia. We sought to generate a novel class of live vaccines that (1) retain all major antigenic virulence proteins yet are fully attenuated and (2) protect against otitis media. A live vaccine candidate based on deletion of the signal recognition pathway component ftsY induced potent, serotype-independent protection against otitis media, sinusitis, pneumonia and invasive pneumococcal disease. Protection was maintained in animals coinfected with influenza virus, but EMBO molecular medicineA live-attenuated pneumococcal vaccine elicits CD4+ T-cell dependent class switching and provides serotype independent protection against acute otitis media.PMC3936495R01 AI027913RC1DC010566RC1 DC010566T32 GM008169T32 AI007401Humann JMurrah KAPerez ACTuomanen EIRosch JWEdward Swords WMann BVogel PGao GMcCullers JAIverson ARMina MfalseA live-attenuated pneumococcal vaccine elicits CD4+ T-cell dependent class switching and provides serotype independent protection against acute otitis media.Acute otitis media (AOM) caused by Streptococcus pneumoniae remains one of the most common infectious diseases worldwide despite widespread vaccination. A major limitation of the currently licensed pneumococcal vaccines is the lack of efficacy against mucosal disease manifestations such as AOM, acute bacterial sinusitis and pneumonia. We sought to generate a novel class of live vaccines that (1) retain all major antigenic virulence proteins yet are fully attenuated and (2) protect against otitis media. A live vaccine candidate based on deletion of the signal recognition pathway component ftsY induced potent, serotype-independent protection against otitis media, sinusitis, pneumonia and invasive pneumococcal disease. Protection was maintained in animals coinfected with influenza virus, but 2014-01-01T00:00:00Z2014 Jan2025-05-18T12:47:20.079Z2025-05-18T12:47:20.079ZS-EPMC39364952440896810.1002/emmm.201202150