{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang LX"],"funding":["FIC NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["3146-51"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3939909"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["111(8)"],"pubmed_abstract":["Lack of an effective small-animal model to study the Kaposi's sarcoma-associated herpesvirus (KSHV) infection in vivo has hampered studies on the pathogenesis and transmission of KSHV. The objective of our study was to determine whether the humanized BLT (bone marrow, liver, and thymus) mouse (hu-BLT) model generated from NOD/SCID/IL2rγ mice can be a useful model for studying KSHV infection. We have tested KSHV infection of hu-BLT mice via various routes of infection, including oral and intravaginal routes, to mimic natural routes of transmission, with recombinant KSHV over a 1- or 3-mo period. Infection was determined by measuring viral DNA, latent and lytic viral transcripts and antigens in various tissues by PCR, in situ hybridization, and immunohistochemical staining. KSHV DNA, as well"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Humanized-BLT mouse model of Kaposi's sarcoma-associated herpesvirus infection."],"pmcid":["PMC3939909"],"funding_grant_id":["P30 GM103509","D43 TW001429","D43TW01492","R01 CA075903","CA75903","GM103509"],"pubmed_authors":["Li Y","Kang G","Lu W","Wood C","Wang LX","Li Q","Kumar P","Zhou Y"],"additional_accession":[]},"is_claimable":false,"name":"Humanized-BLT mouse model of Kaposi's sarcoma-associated herpesvirus infection.","description":"Lack of an effective small-animal model to study the Kaposi's sarcoma-associated herpesvirus (KSHV) infection in vivo has hampered studies on the pathogenesis and transmission of KSHV. The objective of our study was to determine whether the humanized BLT (bone marrow, liver, and thymus) mouse (hu-BLT) model generated from NOD/SCID/IL2rγ mice can be a useful model for studying KSHV infection. We have tested KSHV infection of hu-BLT mice via various routes of infection, including oral and intravaginal routes, to mimic natural routes of transmission, with recombinant KSHV over a 1- or 3-mo period. Infection was determined by measuring viral DNA, latent and lytic viral transcripts and antigens in various tissues by PCR, in situ hybridization, and immunohistochemical staining. KSHV DNA, as well","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Feb","modification":"2026-05-06T21:14:44.422Z","creation":"2019-03-27T01:22:35Z"},"accession":"S-EPMC3939909","cross_references":{"pubmed":["24516154"],"doi":["10.1073/pnas.1318175111"]}}