{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(3)"],"submitter":["Sakurai M"],"pubmed_abstract":["Inosine is an abundant RNA modification in the human transcriptome and is essential for many biological processes in modulating gene expression at the post-transcriptional level. Adenosine deaminases acting on RNA (ADARs) catalyze the hydrolytic deamination of adenosines to inosines (A-to-I editing) in double-stranded regions. We previously established a biochemical method called \"inosine chemical erasing\" (ICE) to directly identify inosines on RNA strands with high reliability. Here, we have applied the ICE method combined with deep sequencing (ICE-seq) to conduct an unbiased genome-wide screening of A-to-I editing sites in the transcriptome of human adult brain. Taken together with the sites identified by the conventional ICE method, we mapped 19,791 novel sites and newly found 1258 edit"],"journal":["Genome research"],"pagination":["522-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3941116"],"repository":["biostudies-literature"],"pubmed_title":["A biochemical landscape of A-to-I RNA editing in the human brain transcriptome."],"pmcid":["PMC3941116"],"pubmed_authors":["Fujiyama A","Suzuki T","Yano T","Toyoda A","Mitsuyama T","Kawabata H","Sakurai M","Terajima H","Okada S","Ueda H"],"additional_accession":[]},"is_claimable":false,"name":"A biochemical landscape of A-to-I RNA editing in the human brain transcriptome.","description":"Inosine is an abundant RNA modification in the human transcriptome and is essential for many biological processes in modulating gene expression at the post-transcriptional level. Adenosine deaminases acting on RNA (ADARs) catalyze the hydrolytic deamination of adenosines to inosines (A-to-I editing) in double-stranded regions. We previously established a biochemical method called \"inosine chemical erasing\" (ICE) to directly identify inosines on RNA strands with high reliability. Here, we have applied the ICE method combined with deep sequencing (ICE-seq) to conduct an unbiased genome-wide screening of A-to-I editing sites in the transcriptome of human adult brain. Taken together with the sites identified by the conventional ICE method, we mapped 19,791 novel sites and newly found 1258 edit","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Mar","modification":"2026-04-29T21:02:10.499Z","creation":"2019-03-27T01:22:42Z"},"accession":"S-EPMC3941116","cross_references":{"pubmed":["24407955"],"doi":["10.1101/gr.162537.113"]}}