biostudies-literatureUnknown24(3)Sakurai MInosine is an abundant RNA modification in the human transcriptome and is essential for many biological processes in modulating gene expression at the post-transcriptional level. Adenosine deaminases acting on RNA (ADARs) catalyze the hydrolytic deamination of adenosines to inosines (A-to-I editing) in double-stranded regions. We previously established a biochemical method called "inosine chemical erasing" (ICE) to directly identify inosines on RNA strands with high reliability. Here, we have applied the ICE method combined with deep sequencing (ICE-seq) to conduct an unbiased genome-wide screening of A-to-I editing sites in the transcriptome of human adult brain. Taken together with the sites identified by the conventional ICE method, we mapped 19,791 novel sites and newly found 1258 editGenome research522-34https://www.ebi.ac.uk/biostudies/studies/S-EPMC3941116biostudies-literatureA biochemical landscape of A-to-I RNA editing in the human brain transcriptome.PMC3941116Fujiyama ASuzuki TYano TToyoda AMitsuyama TKawabata HSakurai MTerajima HOkada SUeda HfalseA biochemical landscape of A-to-I RNA editing in the human brain transcriptome.Inosine is an abundant RNA modification in the human transcriptome and is essential for many biological processes in modulating gene expression at the post-transcriptional level. Adenosine deaminases acting on RNA (ADARs) catalyze the hydrolytic deamination of adenosines to inosines (A-to-I editing) in double-stranded regions. We previously established a biochemical method called "inosine chemical erasing" (ICE) to directly identify inosines on RNA strands with high reliability. Here, we have applied the ICE method combined with deep sequencing (ICE-seq) to conduct an unbiased genome-wide screening of A-to-I editing sites in the transcriptome of human adult brain. Taken together with the sites identified by the conventional ICE method, we mapped 19,791 novel sites and newly found 1258 edit2014-01-01T00:00:00Z2014 Mar2026-04-29T21:02:10.499Z2019-03-27T01:22:42ZS-EPMC39411162440795510.1101/gr.162537.113