biostudies-literatureUnknown14(1)Dahl LD<h4>Background</h4>The oncogene PTI-1 was originally isolated from a prostate cancer cell line by its capability to transform rat fibroblasts. The PTI-1 mRNA has a very eccentric structure as the 5'UTR is similar to prokaryotic 23S rRNA, while the major open reading frame and the 3'UTR corresponds to a part of the mRNA encoding human translation elongation factor eEF1A1. Thus, the largest open reading frame encodes a truncated version of eEF1A1 lacking the first 67 amino acids, while having three unique N-terminal amino acids. Previously, the UTRs were shown to be a prerequisite for the transforming capacity of the PTI-1 transcript. In this study, we have investigated the possible role of the UTRs in regulating protein expression and localization.<h4>Methods</h4>The protein expression profCancer cell international17https://www.ebi.ac.uk/biostudies/studies/S-EPMC3941776biostudies-literatureAn eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus.PMC3941776Nielsen KMFuchtbauer EMCorydon TJKnudsen CRRankel LDahl LDfalseAn eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus.<h4>Background</h4>The oncogene PTI-1 was originally isolated from a prostate cancer cell line by its capability to transform rat fibroblasts. The PTI-1 mRNA has a very eccentric structure as the 5'UTR is similar to prokaryotic 23S rRNA, while the major open reading frame and the 3'UTR corresponds to a part of the mRNA encoding human translation elongation factor eEF1A1. Thus, the largest open reading frame encodes a truncated version of eEF1A1 lacking the first 67 amino acids, while having three unique N-terminal amino acids. Previously, the UTRs were shown to be a prerequisite for the transforming capacity of the PTI-1 transcript. In this study, we have investigated the possible role of the UTRs in regulating protein expression and localization.<h4>Methods</h4>The protein expression prof2014-01-01T00:00:00Z2014 Feb2025-05-18T11:42:01.105Z2025-05-18T11:42:01.105ZS-EPMC39417762457154810.1186/1475-2867-14-17