biostudies-literatureWillett RNIGMS NIH HHSe27888https://www.ebi.ac.uk/biostudies/studies/S-EPMC3948154biostudies-literatureUnknown4(1)The conserved oligomeric Golgi complex is a peripheral membrane protein complex that orchestrates the tethering and fusion of intra-Golgi transport carriers with Golgi membranes. In this study we have investigated the membrane attachment of the COG complex and it's on/off dynamic on Golgi membranes. Several complimentary approaches including knock-sideways depletion, FRAP, and FLIP revealed that assembled COG complex is not diffusing from Golgi periphery in live HeLa cells. Moreover, COG subunits remained membrane-associated even in COG4 and COG7 depleted cells when Golgi architecture was severely affected. Overexpression of myc-tagged COG sub-complexes revealed that different membrane-associated COG partners including β-COP, p115 and SNARE STX5 preferentially bind to different COG assemblCellular logisticsMultipronged interaction of the COG complex with intracellular membranes.PMC3948154R01 GM083144Pokrovskaya ILupashin VKudlyk TWillett RfalseMultipronged interaction of the COG complex with intracellular membranes.The conserved oligomeric Golgi complex is a peripheral membrane protein complex that orchestrates the tethering and fusion of intra-Golgi transport carriers with Golgi membranes. In this study we have investigated the membrane attachment of the COG complex and it's on/off dynamic on Golgi membranes. Several complimentary approaches including knock-sideways depletion, FRAP, and FLIP revealed that assembled COG complex is not diffusing from Golgi periphery in live HeLa cells. Moreover, COG subunits remained membrane-associated even in COG4 and COG7 depleted cells when Golgi architecture was severely affected. Overexpression of myc-tagged COG sub-complexes revealed that different membrane-associated COG partners including β-COP, p115 and SNARE STX5 preferentially bind to different COG assembl2014-01-01T00:00:00Z2014 Jan2025-05-29T19:21:31.297Z2025-05-29T19:21:31.297ZS-EPMC39481542464939510.4161/cl.27888