{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(3)"],"submitter":["Selokar NL"],"pubmed_abstract":["Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apoptotic index, were comparable among embryos produced using cells obtained from fresh or frozen-thawed semen or those obtained from conventional cell sources such as skin. Examination of the epigenetic status revealed that the global level of H3K27me3 but not that of H3K9/14ac and H4K5ac differed significantly (P<0.05) among cloned embryos from different bulls. The relative mRNA abundance of HDAC1, DN"],"journal":["PloS one"],"pagination":["e90755"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3948694"],"repository":["biostudies-literature"],"pubmed_title":["Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen."],"pmcid":["PMC3948694"],"pubmed_authors":["Selokar NL","Saini M","Palta P","Chauhan MS","Manik R","Singla SK"],"additional_accession":[]},"is_claimable":false,"name":"Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen.","description":"Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apoptotic index, were comparable among embryos produced using cells obtained from fresh or frozen-thawed semen or those obtained from conventional cell sources such as skin. Examination of the epigenetic status revealed that the global level of H3K27me3 but not that of H3K9/14ac and H4K5ac differed significantly (P<0.05) among cloned embryos from different bulls. The relative mRNA abundance of HDAC1, DN","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-20T02:46:53.618Z","creation":"2019-03-26T23:24:04Z"},"accession":"S-EPMC3948694","cross_references":{"pubmed":["24614586"],"doi":["10.1371/journal.pone.0090755"]}}