{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stoop EJ"],"funding":["Medical Research Council","Wellcome Trust"],"pagination":["2093-108"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3963455"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(12)"],"pubmed_abstract":["The causative agent of tuberculosis (TB), Mycobacterium tuberculosis, remains an important worldwide health threat. Although TB is one of the oldest infectious diseases of man, a detailed understanding of the mycobacterial mechanisms underlying pathogenesis remains elusive. Here, we studied the role of the α(1→2) mannosyltransferase MptC in mycobacterial virulence, using the Mycobacterium marinum zebrafish infection model. Like its M. tuberculosis orthologue, disruption of M. marinum mptC (mmar_3225) results in defective elongation of mannose caps of lipoarabinomannan (LAM) and absence of α(1→2)mannose branches on the lipomannan (LM) and LAM mannan core, as determined by biochemical analysis (NMR and GC-MS) and immunoblotting. We found that the M. marinum mptC mutant is strongly attenuated"],"journal":["Cellular microbiology"],"pubmed_title":["Mannan core branching of lipo(arabino)mannan is required for mycobacterial virulence in the context of innate immunity."],"pmcid":["PMC3963455"],"funding_grant_id":["081569/Z/06/Z","MR/K012118/1"],"pubmed_authors":["Mishra AK","van der Sar AM","Appelmelk BJ","Bouchier P","Verboom T","van Zon M","Geurtsen J","Driessen NN","Bitter W","Sparrius M","Raadsen SA","van der Wel NN","van Stempvoort G","Stoop EJ","van Leeuwen LM","Besra GS"],"additional_accession":[]},"is_claimable":false,"name":"Mannan core branching of lipo(arabino)mannan is required for mycobacterial virulence in the context of innate immunity.","description":"The causative agent of tuberculosis (TB), Mycobacterium tuberculosis, remains an important worldwide health threat. Although TB is one of the oldest infectious diseases of man, a detailed understanding of the mycobacterial mechanisms underlying pathogenesis remains elusive. Here, we studied the role of the α(1→2) mannosyltransferase MptC in mycobacterial virulence, using the Mycobacterium marinum zebrafish infection model. Like its M. tuberculosis orthologue, disruption of M. marinum mptC (mmar_3225) results in defective elongation of mannose caps of lipoarabinomannan (LAM) and absence of α(1→2)mannose branches on the lipomannan (LM) and LAM mannan core, as determined by biochemical analysis (NMR and GC-MS) and immunoblotting. We found that the M. marinum mptC mutant is strongly attenuated","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Dec","modification":"2026-05-07T10:26:14.045Z","creation":"2026-04-29T03:07:49.446Z"},"accession":"S-EPMC3963455","cross_references":{"pubmed":["23902464"],"doi":["10.1111/cmi.12175"]}}