{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Powers TW"],"funding":["NCI NIH HHS"],"pagination":["9799-806"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3969840"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["85(20)"],"pubmed_abstract":["A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The method has been designed to simultaneously profile the multiple glycan species released from intracellular organelle and cell surface glycoproteins, while maintaining histopathology compatible preparation workflows. A recombinant PNGaseF enzyme was sprayed uniformly across mouse brain tissue slides, incubated for 2 h, then sprayed with 2,5-dihydroxybenzoic acid matrix for MALDI-IMS analysis. Using this b"],"journal":["Analytical chemistry"],"pubmed_title":["Matrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues."],"pmcid":["PMC3969840"],"funding_grant_id":["U01 CA168856","R01 CA104505","R01CA135087","R21CA137704","R01CA104505-05S1","R01 CA120206","R01 CA135087","R01CA104505","R21 CA137704"],"pubmed_authors":["Mehta AS","Gao P","Copland JA","Betesh LR","Powers TW","Jones EE","Romano PR","Drake RR"],"additional_accession":[]},"is_claimable":false,"name":"Matrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues.","description":"A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The method has been designed to simultaneously profile the multiple glycan species released from intracellular organelle and cell surface glycoproteins, while maintaining histopathology compatible preparation workflows. A recombinant PNGaseF enzyme was sprayed uniformly across mouse brain tissue slides, incubated for 2 h, then sprayed with 2,5-dihydroxybenzoic acid matrix for MALDI-IMS analysis. Using this b","dates":{"release":"2013-01-01T00:00:00Z","publication":"2013 Oct","modification":"2025-04-03T23:01:41.679Z","creation":"2019-03-27T01:24:10Z"},"accession":"S-EPMC3969840","cross_references":{"pubmed":["24050758"],"doi":["10.1021/ac402108x"]}}