{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(5)"],"submitter":["Zheng C"],"funding":["Intramural NIH HHS"],"pubmed_abstract":["The promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. The activity from one EF1α promoter (termed EF1α -3), obtained in a commercial vector, was markedly lower when tested in vitro (from 50 - 500 x) in four cell lines and in vivo in rat submandibular glands (~250 x). Sequence differences in the EF1α -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression."],"journal":["International journal of medical sciences"],"pagination":["404-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3970091"],"repository":["biostudies-literature"],"pubmed_title":["All human EF1α promoters are not equal: markedly affect gene expression in constructs from different sources."],"pmcid":["PMC3970091"],"pubmed_authors":["Baum BJ","Zheng C"],"additional_accession":[]},"is_claimable":false,"name":"All human EF1α promoters are not equal: markedly affect gene expression in constructs from different sources.","description":"The promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. The activity from one EF1α promoter (termed EF1α -3), obtained in a commercial vector, was markedly lower when tested in vitro (from 50 - 500 x) in four cell lines and in vivo in rat submandibular glands (~250 x). Sequence differences in the EF1α -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-22T01:01:17.432Z","creation":"2019-03-27T01:24:11Z"},"accession":"S-EPMC3970091","cross_references":{"pubmed":["24688302"],"doi":["10.7150/ijms.8033"]}}