biostudies-literatureUnknown11(5)Zheng CIntramural NIH HHSThe promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. The activity from one EF1α promoter (termed EF1α -3), obtained in a commercial vector, was markedly lower when tested in vitro (from 50 - 500 x) in four cell lines and in vivo in rat submandibular glands (~250 x). Sequence differences in the EF1α -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression.International journal of medical sciences404-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC3970091biostudies-literatureAll human EF1α promoters are not equal: markedly affect gene expression in constructs from different sources.PMC3970091Baum BJZheng CfalseAll human EF1α promoters are not equal: markedly affect gene expression in constructs from different sources.The promoter is a major element in the expression cassette of gene therapy vectors. Optimal promoter selection can enhance target specificity and gene expression. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. The activity from one EF1α promoter (termed EF1α -3), obtained in a commercial vector, was markedly lower when tested in vitro (from 50 - 500 x) in four cell lines and in vivo in rat submandibular glands (~250 x). Sequence differences in the EF1α -3 promoter likely account for the activity differences seen. Investigators need to recognize that all promoters of the same name may not be equivalent in driving transgene expression.2014-01-01T00:00:00Z20142025-04-22T01:01:17.432Z2019-03-27T01:24:11ZS-EPMC39700912468830210.7150/ijms.8033