{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Heiss EH"],"funding":["Austrian Science Fund FWF"],"pagination":["503-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3971763"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["77(3)"],"pubmed_abstract":["Activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) contributes to several beneficial bioactivities of natural products, including induction of an increased cellular stress resistance and prevention or resolution of inflammation. In this study, the potential of a crude leaf extract of Chromolaena odorata, traditionally used against inflammation and skin lesions, was examined for Nrf2 activation. Guided by an Nrf2-dependent luciferase reporter gene assay, the phytoprostane chromomoric acid C-I (1) was identified as a potent Nrf2 activator from C. odorata with a CD (concentration doubling the response of vehicle-treated cells) of 5.2 ?M. When tested at 1-10 ?M, 1 was able to induce the endogenous Nrf2 target gene heme oxygenase 1 (HO-1) in fibroblasts. Between 2 and 5 ?M, compound 1 induced HO-1 in vascular smooth muscle cells (VSMC) and inhibited their proliferation in a HO-1-dependent manner, without eliciting signs of cytotoxicity."],"journal":["Journal of natural products"],"pubmed_title":["Identification of chromomoric acid C-I as an Nrf2 activator in Chromolaena odorata."],"pmcid":["PMC3971763"],"funding_grant_id":["P 23317"],"pubmed_authors":["Vouk C","Dirsch VM","Malainer C","Heiss EH","Atanasov AG","Tran TV","Zimmermann K","Schwaiger S","Mayerhofer B","Stuppner H"],"additional_accession":[]},"is_claimable":false,"name":"Identification of chromomoric acid C-I as an Nrf2 activator in Chromolaena odorata.","description":"Activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) contributes to several beneficial bioactivities of natural products, including induction of an increased cellular stress resistance and prevention or resolution of inflammation. In this study, the potential of a crude leaf extract of Chromolaena odorata, traditionally used against inflammation and skin lesions, was examined for Nrf2 activation. Guided by an Nrf2-dependent luciferase reporter gene assay, the phytoprostane chromomoric acid C-I (1) was identified as a potent Nrf2 activator from C. odorata with a CD (concentration doubling the response of vehicle-treated cells) of 5.2 ?M. When tested at 1-10 ?M, 1 was able to induce the endogenous Nrf2 target gene heme oxygenase 1 (HO-1) in fibroblasts. Between 2 and 5 ?M, compound 1 induced HO-1 in vascular smooth muscle cells (VSMC) and inhibited their proliferation in a HO-1-dependent manner, without eliciting signs of cytotoxicity.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Mar","modification":"2021-03-14T08:52:52Z","creation":"2019-03-27T01:24:15Z"},"accession":"S-EPMC3971763","cross_references":{"pubmed":["24476568"],"doi":["10.1021/np400778m"]}}