{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(4)"],"submitter":["Celik S"],"pubmed_abstract":["The methylation of CpG dinucleotides is a pervasive epigenetic signature with critical roles governing genomic stability and lineage-specific patterns of gene expression. Reprogramming the patterns of CpG methylation accompanies key developmental transitions and the onset of some pathologies, such as cancer. In this study we show that levels of immuno-detectable 5meC decreased as mouse embryonic fibroblasts withdraw from the cell-cycle (became mitotically quiescent), but increased as they aged in culture. Two pools of 5meC epitope were found to exist, one solvent exposed after acid-induced denaturation of chromatin and another that required the additional step of tryptic digestion for detection. Proliferative cells displayed a relatively greater accumulation of detectable 5meC within the t"],"journal":["PloS one"],"pagination":["e92523"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3976252"],"repository":["biostudies-literature"],"pubmed_title":["The exit of mouse embryonic fibroblasts from the cell-cycle changes the nature of solvent exposure of the 5'-methylcytosine epitope within chromatin."],"pmcid":["PMC3976252"],"pubmed_authors":["Celik S","Li Y","O'Neill C"],"additional_accession":[]},"is_claimable":false,"name":"The exit of mouse embryonic fibroblasts from the cell-cycle changes the nature of solvent exposure of the 5'-methylcytosine epitope within chromatin.","description":"The methylation of CpG dinucleotides is a pervasive epigenetic signature with critical roles governing genomic stability and lineage-specific patterns of gene expression. Reprogramming the patterns of CpG methylation accompanies key developmental transitions and the onset of some pathologies, such as cancer. In this study we show that levels of immuno-detectable 5meC decreased as mouse embryonic fibroblasts withdraw from the cell-cycle (became mitotically quiescent), but increased as they aged in culture. Two pools of 5meC epitope were found to exist, one solvent exposed after acid-induced denaturation of chromatin and another that required the additional step of tryptic digestion for detection. Proliferative cells displayed a relatively greater accumulation of detectable 5meC within the t","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-05T00:48:46.887Z","creation":"2020-11-07T09:47:57Z"},"accession":"S-EPMC3976252","cross_references":{"pubmed":["24705067"],"doi":["10.1371/journal.pone.0092523"]}}