biostudies-literatureDawes JMWellcome Truste93338https://www.ebi.ac.uk/biostudies/studies/S-EPMC3986071biostudies-literatureUnknown9(4)Ultraviolet-B (UVB)-induced inflammation produces a dose-dependent mechanical and thermal hyperalgesia in both humans and rats, most likely via inflammatory mediators acting at the site of injury. Previous work has shown that the gene expression of cytokines and chemokines is positively correlated between species and that these factors can contribute to UVB-induced pain. In order to investigate other potential pain mediators in this model we used RNA-seq to perform genome-wide transcriptional profiling in both human and rat skin at the peak of hyperalgesia. In addition we have also measured transcriptional changes in the L4 and L5 DRG of the rat model. Our data show that UVB irradiation produces a large number of transcriptional changes in the skin: 2186 and 3888 genes are significantly dyPloS oneGenome-wide transcriptional profiling of skin and dorsal root ganglia after ultraviolet-B-induced inflammation.PMC398607183259095698Antunes-Martins ASchmid RHildebrandt TGeisslinger GBennett DLMcMahon SBDawes JMPaterson KJPerkins JRSisignano MOrengo CRust WfalseGenome-wide transcriptional profiling of skin and dorsal root ganglia after ultraviolet-B-induced inflammation.Ultraviolet-B (UVB)-induced inflammation produces a dose-dependent mechanical and thermal hyperalgesia in both humans and rats, most likely via inflammatory mediators acting at the site of injury. Previous work has shown that the gene expression of cytokines and chemokines is positively correlated between species and that these factors can contribute to UVB-induced pain. In order to investigate other potential pain mediators in this model we used RNA-seq to perform genome-wide transcriptional profiling in both human and rat skin at the peak of hyperalgesia. In addition we have also measured transcriptional changes in the L4 and L5 DRG of the rat model. Our data show that UVB irradiation produces a large number of transcriptional changes in the skin: 2186 and 3888 genes are significantly dy2014-01-01T00:00:00Z20142026-05-01T19:59:03.088Z2019-03-26T23:24:23ZS-EPMC39860712473296810.1371/journal.pone.0093338