{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(4)"],"submitter":["Christie MP"],"pubmed_abstract":["Glycosylation of biopharmaceuticals can mediate cell specific delivery by targeting carbohydrate receptors. Additionally, glycosylation can improve the physico-chemical (drug-like) properties of peptide based drug candidates. The main purpose of this study was to examine if glycosylation of the peptide enkephalin could facilitate its binding to the carbohydrate receptor, asialoglycoprotein. Firstly, we described the one-pot enzymatic galactosylation of lactose modified enkephalin in the presence of uridine-5'-diphosphogalactose 4-epimerase and lipopolysaccharyl α-1,4-galactosyltransferase. Stability experiments using human plasma and Caco-2 cell homogenates showed that glycosylation considerably improved the stability of enkephalin (at least 60% remained stable after a 2 hr incubation at 3"],"journal":["PloS one"],"pagination":["e95024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3988166"],"repository":["biostudies-literature"],"pubmed_title":["A drug delivery strategy: binding enkephalin to asialoglycoprotein receptor by enzymatic galactosylation."],"pmcid":["PMC3988166"],"pubmed_authors":["Christie MP","Simerska P","Toth I","Hartley-Tassell LE","Jen FE","Jennings MP","Rawi MF","Hussein WM","Day CJ"],"additional_accession":[]},"is_claimable":false,"name":"A drug delivery strategy: binding enkephalin to asialoglycoprotein receptor by enzymatic galactosylation.","description":"Glycosylation of biopharmaceuticals can mediate cell specific delivery by targeting carbohydrate receptors. Additionally, glycosylation can improve the physico-chemical (drug-like) properties of peptide based drug candidates. The main purpose of this study was to examine if glycosylation of the peptide enkephalin could facilitate its binding to the carbohydrate receptor, asialoglycoprotein. Firstly, we described the one-pot enzymatic galactosylation of lactose modified enkephalin in the presence of uridine-5'-diphosphogalactose 4-epimerase and lipopolysaccharyl α-1,4-galactosyltransferase. Stability experiments using human plasma and Caco-2 cell homogenates showed that glycosylation considerably improved the stability of enkephalin (at least 60% remained stable after a 2 hr incubation at 3","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-18T11:33:55.533Z","creation":"2019-03-26T23:24:26Z"},"accession":"S-EPMC3988166","cross_references":{"pubmed":["24736570"],"doi":["10.1371/journal.pone.0095024"]}}