biostudies-literatureUnknown10(4)Katris NJThe apical complex is the definitive cell structure of phylum Apicomplexa, and is the focus of the events of host cell penetration and the establishment of intracellular parasitism. Despite the importance of this structure, its molecular composition is relatively poorly known and few studies have experimentally tested its functions. We have characterized a novel Toxoplasma gondii protein, RNG2, that is located at the apical polar ring--the common structural element of apical complexes. During cell division, RNG2 is first recruited to centrosomes immediately after their duplication, confirming that assembly of the new apical complex commences as one of the earliest events of cell replication. RNG2 subsequently forms a ring, with the carboxy- and amino-termini anchored to the apical polar riPLoS pathogense1004074https://www.ebi.ac.uk/biostudies/studies/S-EPMC3990729biostudies-literatureThe apical complex provides a regulated gateway for secretion of invasion factors in Toxoplasma.PMC3990729Hanssen EKatris NJvan Dooren GGTilley LMcMillan PJWaller RFfalseThe apical complex provides a regulated gateway for secretion of invasion factors in Toxoplasma.The apical complex is the definitive cell structure of phylum Apicomplexa, and is the focus of the events of host cell penetration and the establishment of intracellular parasitism. Despite the importance of this structure, its molecular composition is relatively poorly known and few studies have experimentally tested its functions. We have characterized a novel Toxoplasma gondii protein, RNG2, that is located at the apical polar ring--the common structural element of apical complexes. During cell division, RNG2 is first recruited to centrosomes immediately after their duplication, confirming that assembly of the new apical complex commences as one of the earliest events of cell replication. RNG2 subsequently forms a ring, with the carboxy- and amino-termini anchored to the apical polar ri2014-01-01T00:00:00Z2014 Apr2025-04-05T10:46:46.909Z2019-03-27T01:26:34ZS-EPMC39907292474379110.1371/journal.ppat.1004074