{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["30(4)"],"submitter":["Zhang WY"],"pubmed_abstract":["<h4>Aim</h4>To demonstrate the gene expression profiles mediated by hepatitis B virus X protein (HBx), we characterized the molecular features of pathogenesis associated with HBx in a human liver cell model.<h4>Methods</h4>We examined gene expression profiles in L-O2-X cells, an engineered L-O2 cell line that constitutively expresses HBx, relative to L-O2 cells using an Agilent 22 K human 70-mer oligonucleotide microarray representing more than 21,329 unique, well-characterized Homo sapiens genes. Western blot analysis and RNA interference (RNAi) targeting HBx mRNA validated the overexpression of proliferating cell nuclear antigen (PCNA) and Bcl-2 in L-O2-X cells. Meanwhile, the BrdU incorporation assay was used to test cell proliferation mediated by upregulated cyclooxygenase-2 (COX-2).<h"],"journal":["Acta pharmacologica Sinica"],"pagination":["424-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4002275"],"repository":["biostudies-literature"],"pubmed_title":["Gene expression profiles of human liver cells mediated by hepatitis B virus X protein."],"pmcid":["PMC4002275"],"pubmed_authors":["Xiang R","Shan CL","Zhang WY","Xu FQ","Ye LH","Zhang XD"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression profiles of human liver cells mediated by hepatitis B virus X protein.","description":"<h4>Aim</h4>To demonstrate the gene expression profiles mediated by hepatitis B virus X protein (HBx), we characterized the molecular features of pathogenesis associated with HBx in a human liver cell model.<h4>Methods</h4>We examined gene expression profiles in L-O2-X cells, an engineered L-O2 cell line that constitutively expresses HBx, relative to L-O2 cells using an Agilent 22 K human 70-mer oligonucleotide microarray representing more than 21,329 unique, well-characterized Homo sapiens genes. Western blot analysis and RNA interference (RNAi) targeting HBx mRNA validated the overexpression of proliferating cell nuclear antigen (PCNA) and Bcl-2 in L-O2-X cells. Meanwhile, the BrdU incorporation assay was used to test cell proliferation mediated by upregulated cyclooxygenase-2 (COX-2).<h","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Apr","modification":"2025-04-04T11:10:09.717Z","creation":"2019-08-04T08:20:11Z"},"accession":"S-EPMC4002275","cross_references":{"pubmed":["19343061"],"doi":["10.1038/aps.2009.22"]}}