biostudies-literatureUnknown30(4)Zhang WY<h4>Aim</h4>To demonstrate the gene expression profiles mediated by hepatitis B virus X protein (HBx), we characterized the molecular features of pathogenesis associated with HBx in a human liver cell model.<h4>Methods</h4>We examined gene expression profiles in L-O2-X cells, an engineered L-O2 cell line that constitutively expresses HBx, relative to L-O2 cells using an Agilent 22 K human 70-mer oligonucleotide microarray representing more than 21,329 unique, well-characterized Homo sapiens genes. Western blot analysis and RNA interference (RNAi) targeting HBx mRNA validated the overexpression of proliferating cell nuclear antigen (PCNA) and Bcl-2 in L-O2-X cells. Meanwhile, the BrdU incorporation assay was used to test cell proliferation mediated by upregulated cyclooxygenase-2 (COX-2).<hActa pharmacologica Sinica424-34https://www.ebi.ac.uk/biostudies/studies/S-EPMC4002275biostudies-literatureGene expression profiles of human liver cells mediated by hepatitis B virus X protein.PMC4002275Xiang RShan CLZhang WYXu FQYe LHZhang XDfalseGene expression profiles of human liver cells mediated by hepatitis B virus X protein.<h4>Aim</h4>To demonstrate the gene expression profiles mediated by hepatitis B virus X protein (HBx), we characterized the molecular features of pathogenesis associated with HBx in a human liver cell model.<h4>Methods</h4>We examined gene expression profiles in L-O2-X cells, an engineered L-O2 cell line that constitutively expresses HBx, relative to L-O2 cells using an Agilent 22 K human 70-mer oligonucleotide microarray representing more than 21,329 unique, well-characterized Homo sapiens genes. Western blot analysis and RNA interference (RNAi) targeting HBx mRNA validated the overexpression of proliferating cell nuclear antigen (PCNA) and Bcl-2 in L-O2-X cells. Meanwhile, the BrdU incorporation assay was used to test cell proliferation mediated by upregulated cyclooxygenase-2 (COX-2).<h2009-01-01T00:00:00Z2009 Apr2025-04-04T11:10:09.717Z2019-08-04T08:20:11ZS-EPMC40022751934306110.1038/aps.2009.22