biostudies-literatureUnknown8(1)Liu XThe multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-?) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-? signaling pathway in cell lines.Cellular & molecular immunology88-91https://www.ebi.ac.uk/biostudies/studies/S-EPMC4002985biostudies-literatureIdentification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).PMC4002985Liu XLi HJing XYao ZYang JWang BHe JZhang XDong LWang XGe LfalseIdentification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS).The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-?) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-? signaling pathway in cell lines.2011-01-01T00:00:00Z2011 Jan2020-11-19T08:16:34Z2020-11-19T08:16:34ZS-EPMC40029852092193810.1038/cmi.2010.47