biostudies-literatureUnknown1(8)Kaczmarek PAntiproliferative factor (APF), a sialylated glycopeptide secreted by explanted bladder epithelial cells from interstitial cystitis/painful bladder syndrome (IC/PBS) patients, and its unsialylated analogue (as-APF) significantly decrease proliferation of bladder epithelial cells and/or certain carcinoma cell lines in vitro. We recently reported a structure-activity relationship profile for the peptide portion of as-APF and revealed that truncation of the C-terminal alanine did not significantly affect antiproliferative activity. To better understand the structural basis for the maintenance of activity of this truncated eight amino acid as-APF (as-APF8), we synthesized several amino acid-substituted derivatives and studied their ability to inhibit bladder epithelial cell proliferation in viACS medicinal chemistry letters390-4https://www.ebi.ac.uk/biostudies/studies/S-EPMC4007904biostudies-literatureStructure-Activity Studies on Antiproliferative Factor (APF) Glycooctapeptide Derivatives.PMC4007904Grkovic DTocci GMGuo LZhang COAdams KMBarchi JJKaczmarek PMichejda CJKeay SKKoch KRfalseStructure-Activity Studies on Antiproliferative Factor (APF) Glycooctapeptide Derivatives.Antiproliferative factor (APF), a sialylated glycopeptide secreted by explanted bladder epithelial cells from interstitial cystitis/painful bladder syndrome (IC/PBS) patients, and its unsialylated analogue (as-APF) significantly decrease proliferation of bladder epithelial cells and/or certain carcinoma cell lines in vitro. We recently reported a structure-activity relationship profile for the peptide portion of as-APF and revealed that truncation of the C-terminal alanine did not significantly affect antiproliferative activity. To better understand the structural basis for the maintenance of activity of this truncated eight amino acid as-APF (as-APF8), we synthesized several amino acid-substituted derivatives and studied their ability to inhibit bladder epithelial cell proliferation in vi2010-01-01T00:00:00Z2010 Nov2025-04-05T10:37:31.922Z2019-03-27T01:27:30ZS-EPMC40079042490022310.1021/ml100087a