biostudies-literatureUnknown9(5)Bauer AMembrane envelopment and budding of negative strand RNA viruses (NSVs) is mainly driven by viral matrix proteins (M). In addition, several M proteins are also known to be involved in host cell manipulation. Knowledge about the cellular targets and detailed molecular mechanisms, however, is poor for many M proteins. For instance, Nipah Virus (NiV) M protein trafficking through the nucleus is essential for virus release, but nuclear targets of NiV M remain unknown. To identify cellular interactors of henipavirus M proteins, tagged Hendra Virus (HeV) M proteins were expressed and M-containing protein complexes were isolated and analysed. Presence of acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) in the complex suggested that this protein represents a direct or indirectPloS onee97233https://www.ebi.ac.uk/biostudies/studies/S-EPMC4019565biostudies-literatureANP32B is a nuclear target of henipavirus M proteins.PMC4019565Lamp BNeumann SKarger AKwasnitschka LBauer AHenning AKDietzel EBalkema-Buschmann AFinke SMaisner AKeil GMfalseANP32B is a nuclear target of henipavirus M proteins.Membrane envelopment and budding of negative strand RNA viruses (NSVs) is mainly driven by viral matrix proteins (M). In addition, several M proteins are also known to be involved in host cell manipulation. Knowledge about the cellular targets and detailed molecular mechanisms, however, is poor for many M proteins. For instance, Nipah Virus (NiV) M protein trafficking through the nucleus is essential for virus release, but nuclear targets of NiV M remain unknown. To identify cellular interactors of henipavirus M proteins, tagged Hendra Virus (HeV) M proteins were expressed and M-containing protein complexes were isolated and analysed. Presence of acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) in the complex suggested that this protein represents a direct or indirect2014-01-01T00:00:00Z20142025-04-19T01:31:17.821Z2019-06-06T12:44:39ZS-EPMC40195652482394810.1371/journal.pone.0097233