biostudies-literatureUnknown9Di Bucchianico SGold nanoparticles (Au NPs) are used in many fields, including biomedical applications; however, no conclusive information on their potential cytotoxicity and genotoxicity mechanisms is available. For this reason, experiments in human primary lymphocytes and murine macrophages (Raw264.7) were performed exposing cells to spherical citrate-capped Au NPs with two different nominal diameters (5 nm and 15 nm). The proliferative activity, mitotic, apoptotic, and necrotic markers, as well as chromosomal damage were assessed by the cytokinesis-block micronucleus cytome assay. Fluorescence in situ hybridization with human and murine pancentromeric probes was applied to distinguish between clastogenic and aneuploidogenic effects. Our results indicate that 5 nm and 15 nm Au NPs are able to inhibit ceInternational journal of nanomedicine2191-204https://www.ebi.ac.uk/biostudies/studies/S-EPMC4020889biostudies-literatureAneuploidogenic effects and DNA oxidation induced in vitro by differently sized gold nanoparticles.PMC4020889Valsami-Jones EUboldi CMigliore LGilliland DFabbrizi MRCirillo SDi Bucchianico SfalseAneuploidogenic effects and DNA oxidation induced in vitro by differently sized gold nanoparticles.Gold nanoparticles (Au NPs) are used in many fields, including biomedical applications; however, no conclusive information on their potential cytotoxicity and genotoxicity mechanisms is available. For this reason, experiments in human primary lymphocytes and murine macrophages (Raw264.7) were performed exposing cells to spherical citrate-capped Au NPs with two different nominal diameters (5 nm and 15 nm). The proliferative activity, mitotic, apoptotic, and necrotic markers, as well as chromosomal damage were assessed by the cytokinesis-block micronucleus cytome assay. Fluorescence in situ hybridization with human and murine pancentromeric probes was applied to distinguish between clastogenic and aneuploidogenic effects. Our results indicate that 5 nm and 15 nm Au NPs are able to inhibit ce2014-01-01T00:00:00Z20142025-04-05T10:38:08.018Z2019-03-27T01:28:11ZS-EPMC40208892485535610.2147/IJN.S58397