biostudies-literatureUnknown9(5)Neubauer SThe global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.PloS onee97254https://www.ebi.ac.uk/biostudies/studies/S-EPMC4022651biostudies-literatureSubstitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.PMC4022651Dolgova OPrag GNeubauer SMakarewicz OBorriss RfalseSubstitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.The global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.2014-01-01T00:00:00Z20142021-03-03T08:49:22Z2019-03-26T23:24:49ZS-EPMC40226512483208910.1371/journal.pone.0097254