{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(5)"],"submitter":["Mullin BH"],"pubmed_abstract":["Osteoporosis is a common bone disease that has a strong genetic component. Genome-wide linkage studies have identified the chromosomal region 3p14-p22 as a quantitative trait locus for bone mineral density (BMD). We have previously identified associations between variation in two related genes located in 3p14-p22, ARHGEF3 and RHOA, and BMD in women. In this study we performed knockdown of these genes using small interfering RNA (siRNA) in human osteoblast-like and osteoclast-like cells in culture, with subsequent microarray analysis to identify genes differentially regulated from a list of 264 candidate genes. Validation of selected findings was then carried out in additional human cell lines/cultures using quantitative real-time PCR (qRT-PCR). The qRT-PCR results showed significant down-r"],"journal":["PloS one"],"pagination":["e98116"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4026532"],"repository":["biostudies-literature"],"pubmed_title":["Influence of ARHGEF3 and RHOA knockdown on ACTA2 and other genes in osteoblasts and osteoclasts."],"pmcid":["PMC4026532"],"pubmed_authors":["Mamotte C","Wilson SG","Mullin BH","Prince RL"],"additional_accession":[]},"is_claimable":false,"name":"Influence of ARHGEF3 and RHOA knockdown on ACTA2 and other genes in osteoblasts and osteoclasts.","description":"Osteoporosis is a common bone disease that has a strong genetic component. Genome-wide linkage studies have identified the chromosomal region 3p14-p22 as a quantitative trait locus for bone mineral density (BMD). We have previously identified associations between variation in two related genes located in 3p14-p22, ARHGEF3 and RHOA, and BMD in women. In this study we performed knockdown of these genes using small interfering RNA (siRNA) in human osteoblast-like and osteoclast-like cells in culture, with subsequent microarray analysis to identify genes differentially regulated from a list of 264 candidate genes. Validation of selected findings was then carried out in additional human cell lines/cultures using quantitative real-time PCR (qRT-PCR). The qRT-PCR results showed significant down-r","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-04-04T08:42:21.988Z","creation":"2019-03-26T23:25:40Z"},"accession":"S-EPMC4026532","cross_references":{"pubmed":["24840563"],"doi":["10.1371/journal.pone.0098116"]}}