{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Veloso A"],"funding":["NIEHS NIH HHS","NHGRI NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["896-905"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4032854"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(6)"],"pubmed_abstract":["The rate of transcription elongation plays an important role in the timing of expression of full-length transcripts as well as in the regulation of alternative splicing. In this study, we coupled Bru-seq technology with 5,6-dichlorobenzimidazole 1-β-D-ribofuranoside (DRB) to estimate the elongation rates of over 2000 individual genes in human cells. This technique, BruDRB-seq, revealed gene-specific differences in elongation rates with a median rate of around 1.5 kb/min. We found that genes with rapid elongation rates showed higher densities of H3K79me2 and H4K20me1 histone marks compared to slower elongating genes. Furthermore, high elongation rates had a positive correlation with gene length, low complexity DNA sequence, and distance from the nearest active transcription unit. Features t"],"journal":["Genome research"],"pubmed_title":["Rate of elongation by RNA polymerase II is associated with specific gene features and epigenetic modifications."],"pmcid":["PMC4032854"],"funding_grant_id":["P30 ES017885","R01 HG006786","1R21ES020946","1R01HG006786","P50CA130810","T32 GM007544","P50 CA130810","R21 ES020946"],"pubmed_authors":["Magnuson B","Wilson TE","Biewen B","Veloso A","Paulsen MT","Ljungman M","Kirkconnell KS"],"additional_accession":[]},"is_claimable":false,"name":"Rate of elongation by RNA polymerase II is associated with specific gene features and epigenetic modifications.","description":"The rate of transcription elongation plays an important role in the timing of expression of full-length transcripts as well as in the regulation of alternative splicing. In this study, we coupled Bru-seq technology with 5,6-dichlorobenzimidazole 1-β-D-ribofuranoside (DRB) to estimate the elongation rates of over 2000 individual genes in human cells. This technique, BruDRB-seq, revealed gene-specific differences in elongation rates with a median rate of around 1.5 kb/min. We found that genes with rapid elongation rates showed higher densities of H3K79me2 and H4K20me1 histone marks compared to slower elongating genes. Furthermore, high elongation rates had a positive correlation with gene length, low complexity DNA sequence, and distance from the nearest active transcription unit. Features t","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jun","modification":"2025-04-03T22:06:37.755Z","creation":"2019-06-06T12:48:42Z"},"accession":"S-EPMC4032854","cross_references":{"pubmed":["24714810"],"doi":["10.1101/gr.171405.113"]}}