{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Annaluru N"],"funding":["Wellcome Trust","NIGMS NIH HHS"],"pagination":["55-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4033833"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["344(6179)"],"pubmed_abstract":["Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S"],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Total synthesis of a functional designer eukaryotic chromosome."],"pmcid":["PMC4033833"],"funding_grant_id":["092076","R01 GM077291","GM077291","R01 GM090192"],"pubmed_authors":["Bohutski P","Moore AM","Qiu J","Koszul R","Guo Z","Paul M","DiCarlo JE","Annaluru N","Martin JA","Deng P","Jabbari J","Soh IY","Sotuyo NE","Yu AT","Srinivas V","Bilgel M","Patel R","Gladowski D","McMillan A","Feinberg JI","Yang K","Boulier KM","Capaldo BJ","Doong J","Xie WR","Mitchell LA","Lau CY","Ishizuka I","Caravelli K","Han JS","Li S","Lin D","Dunn J","Kandavelou K","Hadjithomas M","Linder ME","Tullman J","Muller H","Fischer G","Charoen K","Xu Y","Stracquadanio G","McDade JE","Rhee A","Liu J","Chandrasegaran S","Floria CE","Ramalingam S","Paulsen LC","Lee PA","Zeller K","Mao J","Agier N","Yeluru A","Rubashkin MG","Wong A","Agmon N","Murugan S","Oh WC","Chang J","Boeke JD","Bader JS","Wong R","Scheifele LZ","Fernandez C","Ling J","Kuang Z","Cooper EM","Choi WJ","London M","Cirelli K","Richardson SM","Dymond JS","Ma H","Cai Y","London V","Ouyang Y","Hadidi P","Suarez A"],"additional_accession":[]},"is_claimable":false,"name":"Total synthesis of a functional designer eukaryotic chromosome.","description":"Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Apr","modification":"2026-04-29T13:24:11.581Z","creation":"2026-04-07T15:15:12.252Z"},"accession":"S-EPMC4033833","cross_references":{"pubmed":["24674868"],"doi":["10.1126/science.1249252"]}}