{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang CC"],"funding":["NCI NIH HHS","NIH HHS"],"pagination":["345-56"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4035356"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(4)"],"pubmed_abstract":["Basal-like breast carcinoma is characterized by poor prognosis and high intratumour heterogeneity. In an immortalized basal-like breast epithelial cell line, we identified two anticorrelated gene-expression programs that arise among single extracellular matrix (ECM)-attached cells during organotypic three-dimensional culture. The first contains multiple TGF-β-related genes including TGFBR3, whereas the second contains JUND and the basal-like marker KRT5. TGFBR3 and JUND interconnect through four negative-feedback loops to form a circuit that exhibits spontaneous damped oscillations in three-dimensional culture. The TGFBR3-JUND circuit is conserved in some premalignant lesions that heterogeneously express KRT5. The circuit depends on ECM engagement, as detachment causes a rewiring that is t"],"journal":["Nature cell biology"],"pubmed_title":["A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies."],"pmcid":["PMC4035356"],"funding_grant_id":["1-P2-OD006464","DP2 OD006464","P30 CA044579"],"pubmed_authors":["Atkins KA","Bajikar SS","Wang CC","Jamal L","Janes KA"],"additional_accession":[]},"is_claimable":false,"name":"A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.","description":"Basal-like breast carcinoma is characterized by poor prognosis and high intratumour heterogeneity. In an immortalized basal-like breast epithelial cell line, we identified two anticorrelated gene-expression programs that arise among single extracellular matrix (ECM)-attached cells during organotypic three-dimensional culture. The first contains multiple TGF-β-related genes including TGFBR3, whereas the second contains JUND and the basal-like marker KRT5. TGFBR3 and JUND interconnect through four negative-feedback loops to form a circuit that exhibits spontaneous damped oscillations in three-dimensional culture. The TGFBR3-JUND circuit is conserved in some premalignant lesions that heterogeneously express KRT5. The circuit depends on ECM engagement, as detachment causes a rewiring that is t","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Apr","modification":"2026-05-03T11:47:52.599Z","creation":"2019-03-27T01:29:00Z"},"accession":"S-EPMC4035356","cross_references":{"pubmed":["24658685"],"doi":["10.1038/ncb2930"]}}