biostudies-literatureYu CIntramural NIH HHSNIAAA NIH HHS701-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC4042168biostudies-literatureUnknown24(6)Single-cell sequencing is a powerful tool for delineating clonal relationship and identifying key driver genes for personalized cancer management. Here we performed single-cell sequencing analysis of a case of colon cancer. Population genetics analyses identified two independent clones in tumor cell population. The major tumor clone harbored APC and TP53 mutations as early oncogenic events, whereas the minor clone contained preponderant CDC27 and PABPC1 mutations. The absence of APC and TP53 mutations in the minor clone supports that these two clones were derived from two cellular origins. Examination of somatic mutation allele frequency spectra of additional 21 whole-tissue exome-sequenced cases revealed the heterogeneity of clonal origins in colon cancer. Next, we identified a mutated geCell researchDiscovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing.PMC4042168R21 AA022707Zhang FJian MLu YHu XWu WKWang HYu JSung JJHou YWang JWu HFan FBao LZheng HTang SWu RChen YChen RLiang QXu LYang HHe JHe MLi QYao XYang XLi XLi YYu CZhang XXu XZhu SfalseDiscovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing.Single-cell sequencing is a powerful tool for delineating clonal relationship and identifying key driver genes for personalized cancer management. Here we performed single-cell sequencing analysis of a case of colon cancer. Population genetics analyses identified two independent clones in tumor cell population. The major tumor clone harbored APC and TP53 mutations as early oncogenic events, whereas the minor clone contained preponderant CDC27 and PABPC1 mutations. The absence of APC and TP53 mutations in the minor clone supports that these two clones were derived from two cellular origins. Examination of somatic mutation allele frequency spectra of additional 21 whole-tissue exome-sequenced cases revealed the heterogeneity of clonal origins in colon cancer. Next, we identified a mutated ge2014-01-01T00:00:00Z2014 Jun2025-04-21T20:03:51.418Z2019-03-27T01:29:20ZS-EPMC40421682469906410.1038/cr.2014.43