{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gao J"],"funding":["NHLBI NIH HHS"],"pagination":["2364-74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4052267"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["106(11)"],"pubmed_abstract":["Transmural heterogeneities in Na/K pump current (IP), transient outward K(+)-current (Ito), and Ca(2+)-current (ICaL) play an important role in regulating electrical and contractile activities in the ventricular myocardium. Prior studies indicated angiotensin II (A2) may determine the transmural gradient in Ito, but the effects of A2 on IP and ICaL were unknown. In this study, myocytes were isolated from five muscle layers between epicardium and endocardium. We found a monotonic gradient in both Ip and Ito, with the lowest currents in ENDO. When AT1Rs were inhibited, EPI currents were unaffected, but ENDO currents increased, suggesting endogenous extracellular A2 inhibits both currents in ENDO. IP- and Ito-inhibition by A2 yielded essentially the same K0.5 values, so they may both be regul"],"journal":["Biophysical journal"],"pubmed_title":["Autocrine A2 in the T-system of ventricular myocytes creates transmural gradients in ion transport: a mechanism to match contraction with load?"],"pmcid":["PMC4052267"],"funding_grant_id":["HL094910","HL11401","R01 HL085221","HL85221","R01 HL094410","R01 HL111401"],"pubmed_authors":["Potapova IA","Mathias RT","Gao J","Cohen IS","Kim JH","Sun X"],"additional_accession":[]},"is_claimable":false,"name":"Autocrine A2 in the T-system of ventricular myocytes creates transmural gradients in ion transport: a mechanism to match contraction with load?","description":"Transmural heterogeneities in Na/K pump current (IP), transient outward K(+)-current (Ito), and Ca(2+)-current (ICaL) play an important role in regulating electrical and contractile activities in the ventricular myocardium. Prior studies indicated angiotensin II (A2) may determine the transmural gradient in Ito, but the effects of A2 on IP and ICaL were unknown. In this study, myocytes were isolated from five muscle layers between epicardium and endocardium. We found a monotonic gradient in both Ip and Ito, with the lowest currents in ENDO. When AT1Rs were inhibited, EPI currents were unaffected, but ENDO currents increased, suggesting endogenous extracellular A2 inhibits both currents in ENDO. IP- and Ito-inhibition by A2 yielded essentially the same K0.5 values, so they may both be regul","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jun","modification":"2025-04-21T18:57:10.718Z","creation":"2019-03-27T01:29:52Z"},"accession":"S-EPMC4052267","cross_references":{"pubmed":["24896115"],"doi":["10.1016/j.bpj.2014.04.042"]}}