biostudies-literatureKim KYNEI NIH HHSNIDCD NIH HHSNINDS NIH HHS33-47https://www.ebi.ac.uk/biostudies/studies/S-EPMC4060604biostudies-literatureUnknown268Ca(2+) binding protein 1 (CaBP1) and caldendrin are alternatively spliced variants of a subfamily of CaBPs with high homology to calmodulin. Although CaBP1 and caldendrin regulate effectors including plasma membrane and intracellular Ca(2+) channels in heterologous expression systems, little is known about their functions in vivo. Therefore, we generated mice deficient in CaBP1/caldendrin expression (C-KO) and analyzed the expression and cellular localization of CaBP1 and caldendrin in the mouse brain. Immunoperoxidase labeling with antibodies recognizing both CaBP1 and caldendrin was absent in the brain of C-KO mice, but was intense in multiple brain regions of wild-type mice. By Western blot, the antibodies detected two proteins that were absent in the C-KO mouse and consistent in size wNeuroscienceLocalization and expression of CaBP1/caldendrin in the mouse brain.PMC4060604EY020850R01 NS084190DC009433R55 DC009433R01 EY020850NS084190R01 DC009433P30 DC010362P30 EY001730DC010362Liu XLee AHaeseleer FShepherd AKim KYScholl ESfalseLocalization and expression of CaBP1/caldendrin in the mouse brain.Ca(2+) binding protein 1 (CaBP1) and caldendrin are alternatively spliced variants of a subfamily of CaBPs with high homology to calmodulin. Although CaBP1 and caldendrin regulate effectors including plasma membrane and intracellular Ca(2+) channels in heterologous expression systems, little is known about their functions in vivo. Therefore, we generated mice deficient in CaBP1/caldendrin expression (C-KO) and analyzed the expression and cellular localization of CaBP1 and caldendrin in the mouse brain. Immunoperoxidase labeling with antibodies recognizing both CaBP1 and caldendrin was absent in the brain of C-KO mice, but was intense in multiple brain regions of wild-type mice. By Western blot, the antibodies detected two proteins that were absent in the C-KO mouse and consistent in size w2014-01-01T00:00:00Z2014 May2025-04-19T16:37:22.539Z2019-03-27T01:30:21ZS-EPMC40606042463167610.1016/j.neuroscience.2014.02.052