biostudies-literaturePeng DJNCI NIH HHSNIH600-5https://www.ebi.ac.uk/biostudies/studies/S-EPMC4063277biostudies-literatureUnknown394(3)The mechanism of cisplatin resistance in cancer cells is not fully understood. Here, we show that the Akt/mTOR survival pathway plays an important role in cisplatin resistance in human ovarian cancer cells. Specifically, we found that cisplatin treatment activates the Akt/mTOR survival pathway and that inhibition of this pathway by the PI3K inhibitor LY294002 or knockdown of Akt sensitizes ovarian cancer cells to cisplatin. Furthermore, we generated cisplatin-resistant cells and found that resistant cells express a higher level of activated Akt as compared to their cisplatin sensitive counterparts. Importantly, inhibition of Akt or mTOR sensitized resistant cells to cisplatin-induced apoptosis. Taken together, our data indicate that activation of the Akt/mTOR pathway prevents cisplatin-indBiochemical and biophysical research communicationsRole of the Akt/mTOR survival pathway in cisplatin resistance in ovarian cancer cells.PMC4063277R01 CA100073Peng DJZhou JYWu GSWang JfalseRole of the Akt/mTOR survival pathway in cisplatin resistance in ovarian cancer cells.The mechanism of cisplatin resistance in cancer cells is not fully understood. Here, we show that the Akt/mTOR survival pathway plays an important role in cisplatin resistance in human ovarian cancer cells. Specifically, we found that cisplatin treatment activates the Akt/mTOR survival pathway and that inhibition of this pathway by the PI3K inhibitor LY294002 or knockdown of Akt sensitizes ovarian cancer cells to cisplatin. Furthermore, we generated cisplatin-resistant cells and found that resistant cells express a higher level of activated Akt as compared to their cisplatin sensitive counterparts. Importantly, inhibition of Akt or mTOR sensitized resistant cells to cisplatin-induced apoptosis. Taken together, our data indicate that activation of the Akt/mTOR pathway prevents cisplatin-ind2010-01-01T00:00:00Z2010 Apr2025-05-29T15:58:48.38Z2025-05-29T15:58:48.38ZS-EPMC40632772021488310.1016/j.bbrc.2010.03.029