biostudies-literatureHancock DGCanadian Institutes of Health Researche100613https://www.ebi.ac.uk/biostudies/studies/S-EPMC4065045biostudies-literatureUnknown9(6)Dendritic cells (DCs) are critical for regulating CD4 and CD8 T cell immunity, controlling Th1, Th2, and Th17 commitment, generating inducible Tregs, and mediating tolerance. It is believed that distinct DC subsets have evolved to control these different immune outcomes. However, how DC subsets mount different responses to inflammatory and/or tolerogenic signals in order to accomplish their divergent functions remains unclear. Lipopolysaccharide (LPS) provides an excellent model for investigating responses in closely related splenic DC subsets, as all subsets express the LPS receptor TLR4 and respond to LPS in vitro. However, previous studies of the LPS-induced DC transcriptome have been performed only on mixed DC populations. Moreover, comparisons of the in vivo response of two closely rePloS oneA systems biology approach to the analysis of subset-specific responses to lipopolysaccharide in dendritic cells.PMC4065045MOP-74493Ritchie WFjell CDHancock DGHancock REGuy TVFalsafi RShklovskaya EFazekas de St Groth BfalseA systems biology approach to the analysis of subset-specific responses to lipopolysaccharide in dendritic cells.Dendritic cells (DCs) are critical for regulating CD4 and CD8 T cell immunity, controlling Th1, Th2, and Th17 commitment, generating inducible Tregs, and mediating tolerance. It is believed that distinct DC subsets have evolved to control these different immune outcomes. However, how DC subsets mount different responses to inflammatory and/or tolerogenic signals in order to accomplish their divergent functions remains unclear. Lipopolysaccharide (LPS) provides an excellent model for investigating responses in closely related splenic DC subsets, as all subsets express the LPS receptor TLR4 and respond to LPS in vitro. However, previous studies of the LPS-induced DC transcriptome have been performed only on mixed DC populations. Moreover, comparisons of the in vivo response of two closely re2014-01-01T00:00:00Z20142026-05-02T05:36:03.045Z2019-03-26T23:26:16ZS-EPMC40650452494985510.1371/journal.pone.0100613