biostudies-literatureUnknown7Bzdrenga J<h4>Background</h4>SacPox, an enzyme from the extremophilic crenarchaeal Sulfolobus acidocaldarius (Sac), was isolated by virtue of its phosphotriesterase (or paraoxonase; Pox) activity, i.e. its ability to hydrolyze the neurotoxic organophosphorus insecticides. Later on, SacPox was shown to belong to the Phosphotriesterase-Like Lactonase family that comprises natural lactonases, possibly involved in quorum sensing, and endowed with promiscuous, phosphotriesterase activity.<h4>Results</h4>Here, we present a comprehensive and broad enzymatic characterization of the natural lactonase and promiscuous organophosphorus hydrolase activities of SacPox, as well as a structural analysis using a model.<h4>Conclusion</h4>Kinetic experiments show that SacPox is a proficient lactonase, including at rooBMC research notes333https://www.ebi.ac.uk/biostudies/studies/S-EPMC4068969biostudies-literatureSacPox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase.PMC4068969Gotthard GChampion CHiblot JChabriere EBzdrenga JElias MfalseSacPox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase.<h4>Background</h4>SacPox, an enzyme from the extremophilic crenarchaeal Sulfolobus acidocaldarius (Sac), was isolated by virtue of its phosphotriesterase (or paraoxonase; Pox) activity, i.e. its ability to hydrolyze the neurotoxic organophosphorus insecticides. Later on, SacPox was shown to belong to the Phosphotriesterase-Like Lactonase family that comprises natural lactonases, possibly involved in quorum sensing, and endowed with promiscuous, phosphotriesterase activity.<h4>Results</h4>Here, we present a comprehensive and broad enzymatic characterization of the natural lactonase and promiscuous organophosphorus hydrolase activities of SacPox, as well as a structural analysis using a model.<h4>Conclusion</h4>Kinetic experiments show that SacPox is a proficient lactonase, including at roo2014-01-01T00:00:00Z2014 Jun2026-05-04T07:48:34.53Z2019-03-27T01:30:46ZS-EPMC40689692489460210.1186/1756-0500-7-333