{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Holowka D"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["1210-6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4069244"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1841(8)"],"pubmed_abstract":["Polyunsaturated fatty acids (PUFAs) have been found to be effective inhibitors of cell signaling in numerous contexts, and we find that acute addition of micromolar PUFAs such as linoleic acid effectively inhibit of Ca(2+) responses in mast cells stimulated by antigen-mediated crosslinking of FcεRI or by the SERCA pump inhibitor, thapsigargin. In contrast, the saturated fatty acid, stearic acid, with the same carbon chain length as linoleic acid does not inhibit these responses. Consistent with this inhibition of store-operated Ca(2+) entry (SOCE), linoleic acid inhibits antigen-stimulated granule exocytosis to a similar extent. Using the fluorescently labeled plasma membrane Ca(2+) channel protein, AcGFP-Orai1, together with the labeled ER Ca(2+) sensor protein, STIM1-mRFP, we monitor sti"],"journal":["Biochimica et biophysica acta"],"pubmed_title":["Polyunsaturated fatty acids inhibit stimulated coupling between the ER Ca(2+) sensor STIM1 and the Ca(2+) channel protein Orai1 in a process that correlates with inhibition of stimulated STIM1 oligomerization."],"pmcid":["PMC4069244"],"funding_grant_id":["R01AI022449","R01 AI022449","T32 GM008500"],"pubmed_authors":["Baird B","Korzeniowski MK","Bryant KL","Holowka D"],"additional_accession":[]},"is_claimable":false,"name":"Polyunsaturated fatty acids inhibit stimulated coupling between the ER Ca(2+) sensor STIM1 and the Ca(2+) channel protein Orai1 in a process that correlates with inhibition of stimulated STIM1 oligomerization.","description":"Polyunsaturated fatty acids (PUFAs) have been found to be effective inhibitors of cell signaling in numerous contexts, and we find that acute addition of micromolar PUFAs such as linoleic acid effectively inhibit of Ca(2+) responses in mast cells stimulated by antigen-mediated crosslinking of FcεRI or by the SERCA pump inhibitor, thapsigargin. In contrast, the saturated fatty acid, stearic acid, with the same carbon chain length as linoleic acid does not inhibit these responses. Consistent with this inhibition of store-operated Ca(2+) entry (SOCE), linoleic acid inhibits antigen-stimulated granule exocytosis to a similar extent. Using the fluorescently labeled plasma membrane Ca(2+) channel protein, AcGFP-Orai1, together with the labeled ER Ca(2+) sensor protein, STIM1-mRFP, we monitor sti","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Aug","modification":"2025-04-18T21:25:19.771Z","creation":"2019-03-27T01:30:47Z"},"accession":"S-EPMC4069244","cross_references":{"pubmed":["24769339"],"doi":["10.1016/j.bbalip.2014.04.006"]}}