{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Felce JH"],"funding":["Medical Research Council","Wellcome Trust"],"pagination":["L41-3"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4070276"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["106(12)"],"pubmed_abstract":["We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membrane protein, CD86, and two G protein-coupled receptors β2AR and mCannR2, all of which behave as dimers in these conventional assays, exhibit monomeric behavior in an improved competition-based type-3 BRET assay designed to circumvent such artifacts."],"journal":["Biophysical journal"],"pubmed_title":["Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay."],"pmcid":["PMC4070276"],"funding_grant_id":["098274","G0901545"],"pubmed_authors":["Davis SJ","Knox RG","Felce JH"],"additional_accession":[]},"is_claimable":false,"name":"Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.","description":"We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membrane protein, CD86, and two G protein-coupled receptors β2AR and mCannR2, all of which behave as dimers in these conventional assays, exhibit monomeric behavior in an improved competition-based type-3 BRET assay designed to circumvent such artifacts.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jun","modification":"2025-04-18T21:25:33.186Z","creation":"2019-03-27T01:30:49Z"},"accession":"S-EPMC4070276","cross_references":{"pubmed":["24940791"],"doi":["10.1016/j.bpj.2014.04.061"]}}