biostudies-literatureUnknown5(3)Gao B<h4>Unlabelled</h4>Motility is essential for the pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a cell wall-anchored motor using chemical energy. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant differences in the flagellar structures of different bacterial species. The molecular bases for these differences, however, are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. Using next-generation sequencing combined with transposon mutagenesis, we have conducted a comprehensive high-throughput genetic screen in CamBioe01349-14https://www.ebi.ac.uk/biostudies/studies/S-EPMC4073491biostudies-literatureNovel components of the flagellar system in epsilonproteobacteria.PMC4073491Gao BLara-Tejero MGalan JEGoodman ALLefebre MfalseNovel components of the flagellar system in epsilonproteobacteria.<h4>Unlabelled</h4>Motility is essential for the pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a cell wall-anchored motor using chemical energy. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant differences in the flagellar structures of different bacterial species. The molecular bases for these differences, however, are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. Using next-generation sequencing combined with transposon mutagenesis, we have conducted a comprehensive high-throughput genetic screen in Ca2014-01-01T00:00:00Z2014 Jun2026-05-02T10:44:12.338Z2026-04-07T17:53:08.095ZS-EPMC40734912496169310.1128/mBio.01349-14