biostudies-literatureMoyes KMNIGMS NIH HHS161-70https://www.ebi.ac.uk/biostudies/studies/S-EPMC4073896biostudies-literatureUnknown41(2)Our objective was to compare mammary tissue gene expression profiles during a Streptococcus uberis (S. uberis) mastitis challenge between lactating cows subjected to dietary-induced negative energy balance (NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5) to better understand the mechanisms associated with NEB and risk of mastitis during the transition period. The NEB cows were feed-restricted to 60% of calculated net energy for lactation requirements for 7 days, and cows assigned to PEB were fed the same diet for ad libitum intake. Five days after feed restriction, one rear mammary quarter of each cow was inoculated with 5,000 cfu of S. uberis (O140J). At 20 h postinoculation, S. uberis-infected mammary quarters from all cows were biopsied for RNA extraPhysiological genomicsMammary gene expression profiles during an intramammary challenge reveal potential mechanisms linking negative energy balance with impaired immune response.PMC4073896R01 GM068946Moyes KMEverts RERodriguez-Zas SLMorin DELoor JJDrackley JKLewin HAfalseMammary gene expression profiles during an intramammary challenge reveal potential mechanisms linking negative energy balance with impaired immune response.Our objective was to compare mammary tissue gene expression profiles during a Streptococcus uberis (S. uberis) mastitis challenge between lactating cows subjected to dietary-induced negative energy balance (NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5) to better understand the mechanisms associated with NEB and risk of mastitis during the transition period. The NEB cows were feed-restricted to 60% of calculated net energy for lactation requirements for 7 days, and cows assigned to PEB were fed the same diet for ad libitum intake. Five days after feed restriction, one rear mammary quarter of each cow was inoculated with 5,000 cfu of S. uberis (O140J). At 20 h postinoculation, S. uberis-infected mammary quarters from all cows were biopsied for RNA extra2010-01-01T00:00:00Z2010 Apr2026-04-18T15:25:46.742Z2026-04-13T03:06:40.878ZS-EPMC40738962010369810.1152/physiolgenomics.00197.2009