{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Schou J"],"funding":["Novo Nordisk Foundation Center for Protein Research"],"pagination":["e100692"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4074068"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(6)"],"pubmed_abstract":["During mitosis large alterations in cellular structures occur rapidly, which to a large extent is regulated by post-translational modification of proteins. Modification of proteins with the small ubiquitin-related protein SUMO2/3 regulates mitotic progression, but few mitotic targets have been identified so far. To deepen our understanding of SUMO2/3 during this window of the cell cycle, we undertook a comprehensive proteomic characterization of SUMO2/3 modified proteins in mitosis and upon mitotic exit. We developed an efficient tandem affinity purification strategy of SUMO2/3 modified proteins from mitotic cells. Combining this purification strategy with cell synchronization procedures and quantitative mass spectrometry allowed for the mapping of numerous novel targets and their dynamics"],"journal":["PloS one"],"pubmed_title":["Comprehensive identification of SUMO2/3 targets and their dynamics during mitosis."],"pmcid":["PMC4074068"],"funding_grant_id":["PI Jakob Nilsson","PI Jesper Velgaard Olsen"],"pubmed_authors":["Olsen JV","Hayward DG","Nilsson J","Kelstrup CD","Schou J"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive identification of SUMO2/3 targets and their dynamics during mitosis.","description":"During mitosis large alterations in cellular structures occur rapidly, which to a large extent is regulated by post-translational modification of proteins. Modification of proteins with the small ubiquitin-related protein SUMO2/3 regulates mitotic progression, but few mitotic targets have been identified so far. To deepen our understanding of SUMO2/3 during this window of the cell cycle, we undertook a comprehensive proteomic characterization of SUMO2/3 modified proteins in mitosis and upon mitotic exit. We developed an efficient tandem affinity purification strategy of SUMO2/3 modified proteins from mitotic cells. Combining this purification strategy with cell synchronization procedures and quantitative mass spectrometry allowed for the mapping of numerous novel targets and their dynamics","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2025-06-01T00:33:17.585Z","creation":"2025-06-01T00:33:17.585Z"},"accession":"S-EPMC4074068","cross_references":{"pubmed":["24971888"],"doi":["10.1371/journal.pone.0100692"]}}