biostudies-literatureGutierrez-Orozco FNIDCR NIH HHSNCI NIH HHS1226-38https://www.ebi.ac.uk/biostudies/studies/S-EPMC4076011biostudies-literatureUnknown58(6)Ulcerative colitis (UC) is a chronic inflammatory disease of the colon. ?-Mangostin (?-MG), the most abundant xanthone in mangosteen fruit, exerts anti-inflammatory and antibacterial activities in vitro. We evaluated the impact of dietary ?-MG on murine experimental colitis and on the gut microbiota of healthy mice.Colitis was induced in C57BL/6J mice by administration of dextran sulfate sodium (DSS). Mice were fed control diet or diet with ?-MG (0.1%). ?-MG exacerbated the pathology of DSS-induced colitis. Mice fed diet with ?-MG had greater colonic inflammation and injury, as well as greater infiltration of CD3(+) and F4/80(+) cells, and colonic myeloperoxidase, than controls. Serum levels of granulocyte colony-stimulating factor, IL-6, and serum amyloid A were also greater in ?-MG-fed animals than in controls. The colonic and cecal microbiota of healthy mice fed ?-MG but no DSS shifted to an increased abundance of Proteobacteria and decreased abundance of Firmicutes and Bacteroidetes, a profile similar to that found in human UC.?-MG exacerbated colonic pathology during DSS-induced colitis. These effects may be associated with an induction of intestinal dysbiosis by ?-MG. Our results suggest that the use of ?-MG-containing supplements by patients with UC may have unintentional risk.Molecular nutrition & food researchDietary ?-mangostin, a xanthone from mangosteen fruit, exacerbates experimental colitis and promotes dysbiosis in mice.PMC4076011P30 CA016058T32 DE014320Mace TBerman-Booty LDClinton SKGalley JDBailey MTSuksamrarn SChitchumroonchokchai CFailla MLThomas-Ahner JMGutierrez-Orozco FLesinski GBfalseDietary ?-mangostin, a xanthone from mangosteen fruit, exacerbates experimental colitis and promotes dysbiosis in mice.Ulcerative colitis (UC) is a chronic inflammatory disease of the colon. ?-Mangostin (?-MG), the most abundant xanthone in mangosteen fruit, exerts anti-inflammatory and antibacterial activities in vitro. We evaluated the impact of dietary ?-MG on murine experimental colitis and on the gut microbiota of healthy mice.Colitis was induced in C57BL/6J mice by administration of dextran sulfate sodium (DSS). Mice were fed control diet or diet with ?-MG (0.1%). ?-MG exacerbated the pathology of DSS-induced colitis. Mice fed diet with ?-MG had greater colonic inflammation and injury, as well as greater infiltration of CD3(+) and F4/80(+) cells, and colonic myeloperoxidase, than controls. Serum levels of granulocyte colony-stimulating factor, IL-6, and serum amyloid A were also greater in ?-MG-fed animals than in controls. The colonic and cecal microbiota of healthy mice fed ?-MG but no DSS shifted to an increased abundance of Proteobacteria and decreased abundance of Firmicutes and Bacteroidetes, a profile similar to that found in human UC.?-MG exacerbated colonic pathology during DSS-induced colitis. These effects may be associated with an induction of intestinal dysbiosis by ?-MG. Our results suggest that the use of ?-MG-containing supplements by patients with UC may have unintentional risk.2014-01-01T00:00:00Z2014 Jun2020-10-29T13:38:29Z2019-03-27T01:31:04ZS-EPMC40760112466876910.1002/mnfr.201300771