{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Conte I"],"funding":["Telethon"],"pagination":["7793-806"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4081098"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(12)"],"pubmed_abstract":["Vertebrate organogenesis is critically sensitive to gene dosage and even subtle variations in the expression levels of key genes may result in a variety of tissue anomalies. MicroRNAs (miRNAs) are fundamental regulators of gene expression and their role in vertebrate tissue patterning is just beginning to be elucidated. To gain further insight into this issue, we analysed the transcriptomic consequences of manipulating the expression of miR-204 in the Medaka fish model system. We used RNA-Seq and an innovative bioinformatics approach, which combines conventional differential expression analysis with the behavior expected by miR-204 targets after its overexpression and knockdown. With this approach combined with a correlative analysis of the putative targets, we identified a wider set of mi"],"journal":["Nucleic acids research"],"pubmed_title":["The combination of transcriptomics and informatics identifies pathways targeted by miR-204 during neurogenesis and axon guidance."],"pmcid":["PMC4081098"],"funding_grant_id":["TGM11SB2"],"pubmed_authors":["Stupka E","Garcia-Manteiga JM","Avellino R","Emmett W","Sanges R","Davidson NP","Lazarevic D","Marco-Ferreres R","Bockett N","Conte I","Bovolenta P","Merella S","Banfi S","Van Heel D","Migliore C","Carrella S","Meroni G"],"additional_accession":[]},"is_claimable":false,"name":"The combination of transcriptomics and informatics identifies pathways targeted by miR-204 during neurogenesis and axon guidance.","description":"Vertebrate organogenesis is critically sensitive to gene dosage and even subtle variations in the expression levels of key genes may result in a variety of tissue anomalies. MicroRNAs (miRNAs) are fundamental regulators of gene expression and their role in vertebrate tissue patterning is just beginning to be elucidated. To gain further insight into this issue, we analysed the transcriptomic consequences of manipulating the expression of miR-204 in the Medaka fish model system. We used RNA-Seq and an innovative bioinformatics approach, which combines conventional differential expression analysis with the behavior expected by miR-204 targets after its overexpression and knockdown. With this approach combined with a correlative analysis of the putative targets, we identified a wider set of mi","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2025-04-19T08:56:37.46Z","creation":"2019-03-27T01:31:23Z"},"accession":"S-EPMC4081098","cross_references":{"pubmed":["24895435"],"doi":["10.1093/nar/gku498"]}}