{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Villegas VE"],"funding":["China Scholarship Council","AFA Insurance","NIDDK NIH HHS","European Union","Mayo Clinic Cancer Center","Swedish Cancer Fund","National Cancer Institute","NCI NIH HHS","Swedish Research Council","Erasmus Scholarship Program","Swedish Childhood Cancer Foundation"],"pagination":["912-26"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4082767"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(5)"],"pubmed_abstract":["Non-coding RNAs are a complex class of nucleic acids, with growing evidence supporting regulatory roles in gene expression. Here we identify a non-coding RNA located head-to-head with the gene encoding the Glioma-associated oncogene 1 (GLI1), a transcriptional effector of multiple cancer-associated signaling pathways. The expression of this three-exon GLI1 antisense (GLI1AS) RNA in cancer cells was concordant with GLI1 levels. siRNAs knockdown of GLI1AS up-regulated GLI1 and increased cellular proliferation and tumor growth in a xenograft model system. Conversely, GLI1AS overexpression decreased the levels of GLI1, its target genes PTCH1 and PTCH2, and cellular proliferation. Additionally, we demonstrate that GLI1 knockdown reduced GLI1AS, while GLI1 overexpression increased GLI1AS, suppor"],"journal":["Molecular oncology"],"pubmed_title":["Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1."],"pmcid":["PMC4082767"],"funding_grant_id":["R01 CA165076","PROJ12/032","P30 CA015083","Dnr: 130014","CAN 2009/579","K2008-67X-20778-01-3","P30 DK084567","CA165076"],"pubmed_authors":["Ramachandran S","Stahle M","Shimokawa T","Sonkoly E","Annaratone L","Fernandez-Zapico ME","Pivarcsi A","Sapino A","Diao Y","Liapi E","Fernandez-Barrena MG","Zaphiropoulos PG","Burglin TR","Rahman MF","Kapranov P","Ramirez Clavijo S","Villegas VE"],"additional_accession":[]},"is_claimable":false,"name":"Identification of novel non-coding RNA-based negative feedback regulating the expression of the oncogenic transcription factor GLI1.","description":"Non-coding RNAs are a complex class of nucleic acids, with growing evidence supporting regulatory roles in gene expression. Here we identify a non-coding RNA located head-to-head with the gene encoding the Glioma-associated oncogene 1 (GLI1), a transcriptional effector of multiple cancer-associated signaling pathways. The expression of this three-exon GLI1 antisense (GLI1AS) RNA in cancer cells was concordant with GLI1 levels. siRNAs knockdown of GLI1AS up-regulated GLI1 and increased cellular proliferation and tumor growth in a xenograft model system. Conversely, GLI1AS overexpression decreased the levels of GLI1, its target genes PTCH1 and PTCH2, and cellular proliferation. Additionally, we demonstrate that GLI1 knockdown reduced GLI1AS, while GLI1 overexpression increased GLI1AS, suppor","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jul","modification":"2026-05-01T17:37:30.51Z","creation":"2019-03-27T01:31:27Z"},"accession":"S-EPMC4082767","cross_references":{"pubmed":["24726458"],"doi":["10.1016/j.molonc.2014.03.009"]}}