biostudies-literaturePires ESNICHD NIH HHSNCATS NIH HHSFIC NIH HHSNIH HHS1405-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC4091973biostudies-literatureUnknown242(12)<h4>Background</h4>Sperm Acrosomal SLLP1 Binding (SAS1B) protein (ovastacin) is an oolemmal binding partner for the intra-acrosomal sperm protein SLLP1.<h4>Results</h4>Immunohistochemical localization revealed that SAS1B translation is restricted among adult tissues to the ovary and oocytes, SAS1B appearing first in follicles at the primary-secondary transition. Quiescent oocytes within primordial follicles and primary follicles did not stain for SAS1B. Examination of neonatal rat ovaries revealed SAS1B expression first as faint signals in postnatal day 3 oocytes, with SAS1B protein staining intensifying with oocyte growth. Irrespective of animal age or estrus stage, SAS1B was seen only in oocytes of follicles that initiated a second granulosa cell layer. The precise temporal and spatial oDevelopmental dynamics : an official publication of the American Association of AnatomistsSAS1B protein [ovastacin] shows temporal and spatial restriction to oocytes in several eutherian orders and initiates translation at the primary to secondary follicle transition.PMC4091973U01HD60491-2S1P51OD010425U01 HD060491D43TW/HD00654UL1 TR000423R01 HD035523U54 HD029099D43 TW000654P51 OD010425R03 HD055129Flickinger CHerr JCCurnow EHlavin CMandal AKlotz KShetty JIshola-Gbenla KMacnamara EChertihin OKhole ADoerwaldt CHerr AKChamberlain CFeldman SHPires ESfalseSAS1B protein [ovastacin] shows temporal and spatial restriction to oocytes in several eutherian orders and initiates translation at the primary to secondary follicle transition.<h4>Background</h4>Sperm Acrosomal SLLP1 Binding (SAS1B) protein (ovastacin) is an oolemmal binding partner for the intra-acrosomal sperm protein SLLP1.<h4>Results</h4>Immunohistochemical localization revealed that SAS1B translation is restricted among adult tissues to the ovary and oocytes, SAS1B appearing first in follicles at the primary-secondary transition. Quiescent oocytes within primordial follicles and primary follicles did not stain for SAS1B. Examination of neonatal rat ovaries revealed SAS1B expression first as faint signals in postnatal day 3 oocytes, with SAS1B protein staining intensifying with oocyte growth. Irrespective of animal age or estrus stage, SAS1B was seen only in oocytes of follicles that initiated a second granulosa cell layer. The precise temporal and spatial o2013-01-01T00:00:00Z2013 Dec2025-04-04T19:24:12.968Z2019-06-06T13:00:47ZS-EPMC40919732403860710.1002/dvdy.24040