biostudies-literatureUnknown21(8)Tsavachidou-Fenner DMary K. Chapman Fund for Renal Cell Carcinoma Molecular Research, The Mary K. Chapman Foundation<h4>Background</h4>Metastatic renal cell carcinoma (mRCC) patients treated with anti-vascular endothelial growth factor (VEGF) therapies demonstrate promising outcomes but not all patients benefit. Factors that predict response remain to be elucidated.<h4>Patients and methods</h4>Nephrectomy material from 37 patients with mRCC receiving bevacizumab +/- erlotinib was used for protein and gene expression assessment. Protein lysates were subjected to reverse-phase protein array profiling. RNA extracts were used to carry out gene expression microarray-based profiling. Normalized protein and gene expression data were correlated with overall survival (OS) and progression-free survival (PFS) using univariate Cox hazard model and linear regression. Immunoblotting was carried out to validate the reAnnals of oncology : official journal of the European Society for Medical Oncology1599-1606https://www.ebi.ac.uk/biostudies/studies/S-EPMC4092253biostudies-literatureGene and protein expression markers of response to combined antiangiogenic and epidermal growth factor targeted therapy in renal cell carcinoma.PMC4092253Tsavachidou-Fenner DPetillo DJonasch ETeh BTannir NTamboli PMills GBLiu WfalseGene and protein expression markers of response to combined antiangiogenic and epidermal growth factor targeted therapy in renal cell carcinoma.<h4>Background</h4>Metastatic renal cell carcinoma (mRCC) patients treated with anti-vascular endothelial growth factor (VEGF) therapies demonstrate promising outcomes but not all patients benefit. Factors that predict response remain to be elucidated.<h4>Patients and methods</h4>Nephrectomy material from 37 patients with mRCC receiving bevacizumab +/- erlotinib was used for protein and gene expression assessment. Protein lysates were subjected to reverse-phase protein array profiling. RNA extracts were used to carry out gene expression microarray-based profiling. Normalized protein and gene expression data were correlated with overall survival (OS) and progression-free survival (PFS) using univariate Cox hazard model and linear regression. Immunoblotting was carried out to validate the re2010-01-01T00:00:00Z2010 Aug2025-04-26T09:08:48.527Z2019-03-27T01:31:53ZS-EPMC40922532008956610.1093/annonc/mdp600